revelarts
04-12-2024, 06:05 PM
One of several reports like this just here for a bookmark
https://news.rebekahbarnett.com.au/p/dna-contamination-in-covid-vaccines
DNA contamination in Covid vaccines DOES get into human cells, new evidence shows
It also appears that the contamination enters the cell nucleus and integrates with human DNA
DNA contamination in Covid vaccines DOES get into human cells, new evidence shows
It also appears that the contamination enters the cell nucleus and integrates with human DNA
REBEKAH BARNETT
MAR 21, 2024
Regulators and fact checkers claim that plasmid DNA contamination in the mRNA Covid vaccines can’t change your genomic DNA, but new evidence suggests that it actually can.
The fact checkers assert that DNA contamination poses no risk to your genomic DNA because your body will naturally destroy any contaminant DNA before it even gets into the cells.
Even if the contaminant DNA could get into cells, there’s no way it can enter the cell nucleus, where genomic integration events occur, they say.
And even if the contaminant DNA could enter the nucleus, which it can’t, it still couldn’t genomically integrate unless specific enzymes are present, they say.
However, results from independent lab testing conducted on ovarian cancer cell lines show that contaminant DNA from Pfizer’s Covid vaccine not only crossed into the cells, but that it survived multiple cell divisions. This is suggestive that the contaminant DNA is able to transfect (enter) the cell nucleus, and that it integrated with the human cell DNA.
TLDR
1. Scientists claim that Pfizer vaccine contaminant DNA has been detected in ovarian cancer cell line DNA, but they do not yet know if it’s chromosomal (heritable) or extra-chromosomal DNA (not heritable)
2. This is an in vitro (in a lab dish) finding, and needs to be replicated in vivo (in a human patient)
3. As the finding is specific to cancer cell lines, it is not generalisable, but scientists say it may give an indication of what cancer patients in remission could experience after mRNA Covid vaccination
4. This finding calls into question fact checker claims that mRNA Covid vaccine DNA contamination can't enter cells, can't enter the nucleus, and cannot integrate with human DNA.
Last year, Boston-based genomics scientist Kevin McKernan made the shocking discovery that the mRNA Covid vaccines are contaminated with excessive levels of plasmid DNA, an artefact of the vaccine production process.
McKernan’s findings were soon confirmed by multiple independent labs around the world for both the Pfizer and Moderna mono- and bi-valent vaccines, including lots approved for children, with one Canadian study led by Dr David Speicher concluding that there are “billions to hundreds of billions of DNA molecules per dose.”
Scientists including McKernan, University of South Carolina cancer genomics scientist Dr Phillip Buckhaults, and Dr Wafik El-Diery, head of the Cancer Centre at Brown University, expressed concerns that fragments of plasmid DNA contamination could cause adverse events, autoimmune problems and cancers in some patients.
But perhaps most significantly, there is also a theoretical risk of the contaminant DNA integrating with patients’ chromosomal DNA and modifying the human genome. This is of particular concern with the Pfizer vaccine, which contains an SV40 enhancer sequence, used in gene therapies “to drive DNA into the nucleus,” explains McKernan.
While regulators have taken a ‘wait and see’ approach, independent scientists, including McKernan, have been more proactive, initiating experiments testing for evidence of genomic integration.
Now, the first results are in.
In an experiment conducted together with German molecular biologist Dr Ulrike Kämmerer, McKernan has detected vaccine contaminant DNA in ovarian cancer cell lines treated with Pfizer’s Covid vaccine.
The scientists found a chimeric combination of human ovarian cell line DNA and spike sequence DNA derived from the contaminating plasmid at at least one, and possibly two sites.
“If anything, this work has put to bed the question regarding if this contaminant DNA gets into the cell, and the chimeric human and contaminant spike DNA sequences imply it has entered the nucleus,” McKernan says.
“The PCR and sequencing data both demonstrate the vaccine is getting into the cell and surviving cell passaging. It is likely bioactive and being partially replicated.”
To reach this finding, Dr Kämmerer first treated ovarian cancer cell lines with mRNA Covid vaccines, using cells treated with AstraZeneca and Janssen vaccines as controls.
The cells were then ‘passaged’, meaning they were left to divide and replicate numerous times. This has the effect of “rinsing away residual vaccine,” explains McKernan.
Immunohistochemistry (IHC) was then performed, a staining process that Dr Kämmerer used to detect levels of spike protein expression produced by the vaccine modified-RNA.
This was to confirm that the lipid nanoparticles (LNPs) carrying mod-RNA and plasmid DNA contamination “did their job and delivered the payload,” says McKernan. Measuring how many cells expressed spike protein also allowed the scientists to determine how much of the vaccine to treat the cells with.
https://news.rebekahbarnett.com.au/p/dna-contamination-in-covid-vaccines
DNA contamination in Covid vaccines DOES get into human cells, new evidence shows
It also appears that the contamination enters the cell nucleus and integrates with human DNA
DNA contamination in Covid vaccines DOES get into human cells, new evidence shows
It also appears that the contamination enters the cell nucleus and integrates with human DNA
REBEKAH BARNETT
MAR 21, 2024
Regulators and fact checkers claim that plasmid DNA contamination in the mRNA Covid vaccines can’t change your genomic DNA, but new evidence suggests that it actually can.
The fact checkers assert that DNA contamination poses no risk to your genomic DNA because your body will naturally destroy any contaminant DNA before it even gets into the cells.
Even if the contaminant DNA could get into cells, there’s no way it can enter the cell nucleus, where genomic integration events occur, they say.
And even if the contaminant DNA could enter the nucleus, which it can’t, it still couldn’t genomically integrate unless specific enzymes are present, they say.
However, results from independent lab testing conducted on ovarian cancer cell lines show that contaminant DNA from Pfizer’s Covid vaccine not only crossed into the cells, but that it survived multiple cell divisions. This is suggestive that the contaminant DNA is able to transfect (enter) the cell nucleus, and that it integrated with the human cell DNA.
TLDR
1. Scientists claim that Pfizer vaccine contaminant DNA has been detected in ovarian cancer cell line DNA, but they do not yet know if it’s chromosomal (heritable) or extra-chromosomal DNA (not heritable)
2. This is an in vitro (in a lab dish) finding, and needs to be replicated in vivo (in a human patient)
3. As the finding is specific to cancer cell lines, it is not generalisable, but scientists say it may give an indication of what cancer patients in remission could experience after mRNA Covid vaccination
4. This finding calls into question fact checker claims that mRNA Covid vaccine DNA contamination can't enter cells, can't enter the nucleus, and cannot integrate with human DNA.
Last year, Boston-based genomics scientist Kevin McKernan made the shocking discovery that the mRNA Covid vaccines are contaminated with excessive levels of plasmid DNA, an artefact of the vaccine production process.
McKernan’s findings were soon confirmed by multiple independent labs around the world for both the Pfizer and Moderna mono- and bi-valent vaccines, including lots approved for children, with one Canadian study led by Dr David Speicher concluding that there are “billions to hundreds of billions of DNA molecules per dose.”
Scientists including McKernan, University of South Carolina cancer genomics scientist Dr Phillip Buckhaults, and Dr Wafik El-Diery, head of the Cancer Centre at Brown University, expressed concerns that fragments of plasmid DNA contamination could cause adverse events, autoimmune problems and cancers in some patients.
But perhaps most significantly, there is also a theoretical risk of the contaminant DNA integrating with patients’ chromosomal DNA and modifying the human genome. This is of particular concern with the Pfizer vaccine, which contains an SV40 enhancer sequence, used in gene therapies “to drive DNA into the nucleus,” explains McKernan.
While regulators have taken a ‘wait and see’ approach, independent scientists, including McKernan, have been more proactive, initiating experiments testing for evidence of genomic integration.
Now, the first results are in.
In an experiment conducted together with German molecular biologist Dr Ulrike Kämmerer, McKernan has detected vaccine contaminant DNA in ovarian cancer cell lines treated with Pfizer’s Covid vaccine.
The scientists found a chimeric combination of human ovarian cell line DNA and spike sequence DNA derived from the contaminating plasmid at at least one, and possibly two sites.
“If anything, this work has put to bed the question regarding if this contaminant DNA gets into the cell, and the chimeric human and contaminant spike DNA sequences imply it has entered the nucleus,” McKernan says.
“The PCR and sequencing data both demonstrate the vaccine is getting into the cell and surviving cell passaging. It is likely bioactive and being partially replicated.”
To reach this finding, Dr Kämmerer first treated ovarian cancer cell lines with mRNA Covid vaccines, using cells treated with AstraZeneca and Janssen vaccines as controls.
The cells were then ‘passaged’, meaning they were left to divide and replicate numerous times. This has the effect of “rinsing away residual vaccine,” explains McKernan.
Immunohistochemistry (IHC) was then performed, a staining process that Dr Kämmerer used to detect levels of spike protein expression produced by the vaccine modified-RNA.
This was to confirm that the lipid nanoparticles (LNPs) carrying mod-RNA and plasmid DNA contamination “did their job and delivered the payload,” says McKernan. Measuring how many cells expressed spike protein also allowed the scientists to determine how much of the vaccine to treat the cells with.